We are proud to offer an extensive menu of clinical tests and services through our four CLIA-certified, CAP-accredited laboratories at Moffitt-Long Hospital, Mission Bay Hospitals, Mt. How to determine and define your hospitals patient glycemic goals Identify problems with high/low glucose levels Learn UCSFs approach to managing and. We additionally have two limited service moderate complexity CLIA-certified. Worthington Tissue Dissociation Guide: Link to website Protocols and Recommendations Cell Harvesting and Preparation Do you have any requests? Submit your suggestion here: SUGGESTION BOX! Other coming: Recommended Sorting supplies, Guidelines for plate sorting. Protein Prospector - UCSF Mass Spectrometry Facility Skyline from the MaCoss laboratory PNNL Proteomics Research Center for Integrative Biology software. UCSF’s innovative, collaborative approach to patient care, research and education spans disciplines across the life sciences, making it a world leader in scientific discovery and its translation to improving health. OMIPs: check this published Optimized Multicolor Immunofluorescence Panels for flow cytometry. ![]() ![]() BioLegend Technical Protocols: check here several standard staining protocols, aided with video tutorials.īio-Protocol 2015: "Intracellular Cytokine Staining on PBMCs Using CyTOF Mass Cytometry"īio-Protocol 2015: "Cytokine-stimulated Phosphoflow of PBMC Using CyTOF Mass Cytometry"īio-Protocol 2015: "Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry" These can provide you a starting point and alleviate the development time in need of the same or highly similar panels.Ĭurrent Protocols in Immunology: research published protocols in this journal, and other Current Protocols multidisciplinary collections. RNA isolation from sorted cells - This paper has a detailed protocol for how to properly prepare a sorter for RNA isolation and sort cells with Trizol, RLT, and RNase inhibitor. It also includes an estimated yield of RNA from certain cell types.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |